Regulation of glucagon secretion by glucose transporter type 2 (glut2) and astrocyte-dependent glucose sensors
J. Clin. Invest. Nell Marty, et al. 115:3545 doi:10.1172/JCI26309 [Go to this article.]

Figure 2
c-FLI cells in the NTS, the DMNX, and the VMH following 2-DG injections. Mice were injected i.p. with NaCl or 2-DG, and tissues were processed 2 hours later for c-FLI immunohistochemistry. (A) Representative photos from c-FLI–positive cells at 2 different levels of the NTS and DMNX of ripglut1;glut2^+/– mice after i.p. injection of NaCl or 2-DG. The positions of the sections were between –7.64 and –7.48 mm relative to the bregma. Scale bars: 100 μm. AP, area postrema; cc, central canal. (B) c-FLI–positive cells in the NTS and DMNX of ripglut1;glut2^+/–, ripglut1;glut2^–/–, and pgfapglut2;ripglut1;glut2^–/– mice after i.p. injection of NaCl or 2-DG. In glut2-null mice, there is no increase in the number of c-FLI–positive cells after 2-DG administration. Transgenic expression of GLUT2 in astrocytes restored the sensitivity of the NTS and DMNX to i.p. 2-DG. (C) c-FLI–positive cells in the VMH of control, ripglut1;glut2^–/–, and pgfapglut2;ripglut1;glut2^–/– mice after i.p. injection of NaCl or 2-DG. Data are indicated as mean ± SD; n = 6–8 mice for each data point. *P < 0.05, **P < 0.01 for comparison between NaCl- and 2-DG–injected groups (Student’s t test).