Regulation of glucagon secretion by glucose transporter type 2 (glut2) and astrocyte-dependent glucose sensors
J. Clin. Invest. Nell Marty, et al. 115:3545 doi:10.1172/JCI26309 [Go to this article.]

Figure 1
Suppression of glucagon secretion in response to physiological hypoglycemia or cellular glucoprivation in ripglut1;glut2^–/– mice. (A) Glycemic profiles during use of normoglycemic or hypoglycemic clamps in ripglut1;glut2^+/– and ripglut1;glut2^–/– mice. (B) Plasma glucagon levels measured at the end of the clamp experiment. Hypoglycemia induced an approximately 3-fold increase in plasma glucagon in control mice but no increase in ripglut1;glut2^–/– mice. (C) Glucagon levels 30 minutes after i.p. injection of NaCl or 2-DG. 2-DG induced an approximately 1.7-fold increase in plasma glucagon in control mice but no increase in glut2-null mice. (D) Glucagon levels measured 30 minutes following i.c.v. injection of NaCl or 2-DG. 2-DG induced a 2.5-fold increase in plasma glucagon in control mice and no increase in ripglut1;glut2^–/– mice. (B and D) Data are indicated as mean ± SD; n = 6–10 for each data point. (C) Data are indicated as mean ± SEM of 3 experiments, each performed with 6–8 mice. **P < 0.01 for comparison between NaCl- and 2-DG–injected groups. ^#P < 0.05 and ^##P < 0.01 for comparison between NaCl–injected control and ripglut1;glut2^–/– groups (Student’s t test).