CD4⁺ T cell–independent DNA vaccination against opportunistic infections
J. Clin. Invest. Mingquan Zheng, et al. 115:3536 doi:10.1172/JCI26306 [Go to this article.]

Figure 1
Humoral responses after pOVA or pOVA/CD40L DNA vaccination. (A) Schema of prime-boost vaccination protocol. DNA vectors used were pOVA, pOVA/CD40L, pBUDCE4 (as empty vector), or pCD40L. Adenovirus boosting was carried out with AdOVA, AdOVA and AdCD40L, AdLuc, or AdLuc and AdCD40L. Anti-OVA IgG2a (B) and IgG1 (C) responses after 3 successive intramuscular DNA vaccinations with pOVA or pOVA/CD40L in CD4-depleted and CD4-replete mice n = 6–8 per group. All data are mean titer ± SEM. *P < 0.05 compared with control. A line denotes the limit of detection in the ELISA.