GDNF rescues hyperglycemia-induced diabetic enteric neuropathy through
activation of the PI3K/Akt pathway
J. Clin. Invest. Mallappa Anitha, et al. 116:344 doi:10.1172/JCI26295 [
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Figure 7Diabetes is associated with decreased expression of PI3K in enteric neurons. Frozen cross sections of control (
A) and STZ-induced diabetic mouse ilea (
B) were assessed for myenteric neuronal p-Akt staining using an antibody specific for p-Akt (red). Arrows point to the myenteric ganglion stained with peripherin (green). The yellow staining represents p-Akt expression due to colocalization with peripherin. (
C) The percentage of p-Akt–positive/peripherin-positive ganglia per section is shown. A total of 3 independent experiments was performed. (
D) Representative Western blot of p-Akt and total Akt assessed in myenteric neurons isolated from the ilea of diabetic and control mice. (
E) Ratio of p-Akt to Akt in myenteric neurons isolated from ilea of diabetic and control mice and assessed by Western blot analysis. A total of 3 independent experiments was performed. *
P < 0.05; ***
P < 0.001. Scale bars: 100 μm.
