GDNF rescues hyperglycemia-induced diabetic enteric neuropathy through activation of the PI3K/Akt pathway
J. Clin. Invest. Mallappa Anitha, et al. 116:344 doi:10.1172/JCI26295 [Go to this article.]

Figure 3
Hyperglycemia is associated with increased FOXO3a translocation. Enteric neurons were cultured in medium containing 5 or 20 mM glucose in the presence or absence of GDNF. Cells were cultured for 24 hours, fixed, and stained for FOXO3a and the neuronal marker peripherin. (A) The percentage of neurons with nuclear translocation of FOXO3a under the different culture conditions is shown. (B) Representative photographs of FOXO3a/peripherin staining. Arrows represent neurons with nuclear translocation of FOXO3a. Results are from 4 independent experiments. *P < 0.05; ***P < 0.001. Scale bars: 20 μm.