Decreased vascular lesion formation in mice with inducible endothelial-specific expression of protein kinase Akt
J. Clin. Invest. Yasushi Mukai, et al. 116:334 doi:10.1172/JCI26223 [Go to this article.]

Figure 6
Immunostaining for cleaved (activated) caspase-3 in ligated carotid arteries. (A) Double-immunofluorescent staining in ligated carotid arteries showing ECs positively stained for cleaved caspase-3 (C. cas-3; green). Staining for PECAM-1 (red) was used to detect and assess the integrity of the endothelial layer. Scale bar: 50 μm. Arrowheads indicate positively stained ECs. L, lumen; A, adventitia. (B) The number of positive-staining cells in 4 separate sections per animal is quantified. Results are expressed as mean ± SEM. n = 4 in each group. *P < 0.05 versus WT.