Decreased vascular lesion formation in mice with inducible endothelial-specific expression of protein kinase Akt
J. Clin. Invest. Yasushi Mukai, et al. 116:334 doi:10.1172/JCI26223 [Go to this article.]

Figure 1
Endothelial-specific expression of inducible myrAkt transgene in EC-Akt Tg mice. (A) Western blots in primary MLEC cultures. The expression of myrAkt transgene was determined by the expression of HA tag. ECs from EC-Akt Tg mice were divided into 2 groups, with and without myrAkt gene induction. Phosphorylated forms of Akt, GSK-3, or eNOS were determined in these ECs. Each blot is representative of 3 independent experiments. (B) Endothelial expression of myrAkt in vivo. Immunostaining in carotid artery, showing HA-tag expression in the endothelial layer of an induced EC-Akt Tg mouse. Scale bar: 50 μm. (C) Immunoblots showing phosphorylation of Akt in aortae from induced EC-Akt Tg mice (left) and in primary cultures of aortic SMCs isolated from induced EC-Akt Tg mice (MASMC, right).