Shotgun proteomics implicates protease inhibition and complement activation in the antiinflammatory properties of HDL
J. Clin. Invest. Tomas Vaisar, et al. 117:746 doi:10.1172/JCI26206 [
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Figure 7Relationship between unique peptides and protein concentration in HDL and HDL
3.
The total number of unique peptides detected by MS in HDL and HDL
3 is as shown in Table
2. The protein concentrations of apoA-I (98 mg/dl), apoA-II (42 mg/dl), apoC-II (4.6 mg/dl), apoB (1.7 mg/dl), and transferrin (0.02 mg/dl) in HDL
3 and HDL isolated by ultracentrifugation are those reported by McPherson et al. (where HDL equals the sum of HDL
2 and HDL
3; ref.
41). The concentration of CETP in HDL
3 (0.1 mg/dl) was estimated as 50% that of plasma CETP (
40). Linear regression analysis revealed a strong correlation (
r2 = 0.98 and 0.88) between protein concentration and unique peptides in HDL and HDL
3, respectively.
