SOCS1 restricts dendritic cells’ ability to break self tolerance and induce antitumor immunity by regulating IL-12 production and signaling
J. Clin. Invest. Kevin Evel-Kabler, et al. 116:90 doi:10.1172/JCI26169 [Go to this article.]

Figure 5
SOCS1 restricts the level and duration of IL-12 produced by DCs. (A and B) Levels of IL-12p40 (A) and IL-12p70 (B) secreted by DCs (5 × 10⁵ cells/ml) in response to continuous stimulation with LPS (100 ng/ml) with or without plate-coated anti-CD40 mAb (1:1,000 dilution) for 24 hours from 1 of 3 independent experiments. All BM DCs were cultured with mGM-CSF (20 ng/ml) in the presence or absence of mIL-4 (20 ng/ml). *P < 0.01 versus SOCS1-siRNA DCs. (C). Kinetics of IL-12p70 production by DCs (5 × 10⁵ cells/ml) in response to continuous stimulation with LPS (100 ng/ml) and anti-CD40 mAb for 0–72 hours from 1 of 3 independent experiments. All cells were cultured in the presence of both GM-CSF and IL-4. *P < 0.01 GFP-siRNA DCs versus SOCS1-siRNA DCs.