The H1 histamine receptor regulates allergic lung responses
J. Clin. Invest. Paul J. Bryce, et al. 116:1624 doi:10.1172/JCI26150 [Go to this article.]

Figure 4
OVA-specific T cells migrate toward histamine via an H1R-dependent process. Chemotaxis to histamine was investigated using the OVA-specific DO11.10 T cell hybridoma. (A) DO11.10 cells migrate in response to histamine. Cells were added to the upper chamber of Transwell chambers, and histamine was added to the lower chamber. After 4 hours, the number of cells in the lower chamber was determined by FACS. (B) Diphenhydramine (DPH), an H1R antagonist; ranitidine (RAN), an H2R antagonist; and thioperamide (THIOP), an H3R/H4R antagonist, were added to assess their effects on T cell chemotaxis induced by histamine (100 μM). Data represent the number of cells moving through Transwell chambers (mean cells ± SEM) from 3 separate experiments. **P < 0.01 by Student’s t test.