Suppression of oxidative metabolism and mitochondrial biogenesis by the transcriptional corepressor RIP140 in mouse adipocytes
J. Clin. Invest. Aimee M. Powelka, et al. 116:125 doi:10.1172/JCI26040 [
Go to this article.]
Figure 3Insulin signaling is not significantly affected by RIP140 silencing. Four days after the induction of differentiation, 3T3-L1 adipocytes were transfected with scrambled or RIP140 siRNA. Three days later, the cells were starved for 2 hours and then stimulated with insulin for 30 minutes. Cell lysates were used for total and phospho-Akt and MAPK blots. The representative blot shows a small but insignificant increase in phospho-serine Akt levels.
