Suppression of oxidative metabolism and mitochondrial biogenesis by the transcriptional corepressor RIP140 in mouse adipocytes
J. Clin. Invest. Aimee M. Powelka, et al. 116:125 doi:10.1172/JCI26040 [Go to this article.]

Figure 2
RIP140 depletion enhances GLUT4 expression and hexose uptake in both day-4 and day-8 3T3-L1 adipocytes. Four (day 4) or eight (day 8) days after the induction of differentiation, 3T3-L1 adipocytes were transfected with scrambled or RIP140 siRNA. Three days later, cell lysates were used for SDS-PAGE to assess GLUT4, GLUT1, and actin expression levels. Intact cells were used to examine the effect of RIP140 depletion on 2-deoxyglucose uptake. The graph shows the mean ± SEM of 5 independent experiments. *P < 0.05 compared with scrambled, by Student’s t test.