Tbx18 regulates the development
of the ureteral mesenchyme
J. Clin. Invest. Rannar Airik, et al. 116:663 doi:10.1172/JCI26027 [
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Figure 4Mislocalization of
Tbx18:LacZ expression in
Tbx18–/– urogenital systems. (
A–
E) β-Galactosidase activity staining of a
LacZ reporter gene in the
Tbx18 locus was analyzed in kidneys at E11.5 (
A), in urogenital systems at E12.5 (
B) and E14.5 (
D), and in transverse sections of E12.5 (
C) and E14.5 kidneys (
E). P1 and P2 in
C indicate sections from the planes shown in B. P1, P2, and P3 in
E indicate section planes shown in
D. P2high is a higher magnification of P2 in the region of the ureter. Black arrows indicate the ureteric epithelium. (
F and
G) Hematoxylin and eosin stainings of transverse sections of E14.5 kidneys. Yellow arrows indicate the renal capsule. The boxed regions in
F are magnified in
G. (
H) β-Galactosidase activity staining of 2-day-old cultures of metanephric rudiments that were wild type as well as heterozygous and homozygous for the mutant
Tbx18LacZ allele. Slight blue staining of the ureteric epithelium in wild-type mice was due to endogenous β-galactosidase activity. Heterozygotes and homozygotes in
A,
B, and
D were
Tbx18LacZ/+ and
Tbx18LacZ/Tbx18LacZ, respectively, whereas embryos used for
C and
E were normalized for the
LacZ allele (i.e.,
Tbx18LacZ/+ and
Tbx18/Tbx18LacZ, respectively). cm, condensing (metanephric) mesenchyme. Arrowheads in
A and
C–
H mark localization of
Tbx18-positive prospective and definitive ureteral mesenchymal cells in kidneys, urogenital systems, and kidney cultures at the indicated stages. Scale bars: 100 μm (
A,
C, and
E–
H), 1,000 μm (
B and
D).
