Mice lacking ghrelin receptors resist
the development of diet-induced obesity
J. Clin. Invest. Jeffrey M. Zigman, et al. 115:3564 doi:10.1172/JCI26002 [
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Figure 1Gene targeting of the
Ghsr locus. Upper left panel: Schematic diagram of the derivation of GHSR-null mice by homologous recombination. Lower left panel: Southern blot analysis of genomic DNA extracted from representative progeny of mating animals heterozygous for the recombinant
Ghsr allele. Right panels: Representative dark-field photomicrographs of in situ hybridization histochemistry experiments performed on mouse brains using a mouse GHSR–specific riboprobe.
Ghsr mRNA expression is evidenced by the white-appearing silver granules. 1–3,
Ghsr exons 1–3; 3V, third ventricle; AP, area postrema; Arc, arcuate nucleus; DMV, dorsal motor nucleus of the vagus; NTS, nucleus of the solitary tract; P, Southern probe. Scale bar: 200 μm (applies to all 4 panels).
