Drusen deposits associated with aging and age-related macular degeneration contain nonfibrillar amyloid oligomers
J. Clin. Invest. Volker Luibl, et al. 116:378 doi:10.1172/JCI25843 [Go to this article.]

Figure 3
ELISA of retinal extracts using the A11 anti-oligomer antibody. (A) Increasing amounts of oligomers made from the Aβ_1–40 peptide resulted in a dose-dependent response when incubated with the A11 anti-oligomer–specific antibody (filled circles). Little or no reactivity was observed when the Aβ_1–40 oligomers were incubated without the primary antibody (open circles). (B) Dose-dependent reactivity was observed when the A11 antibody was incubated with increasing amounts of extract prepared from dissected drusen/RPE/Bm tissue from a 76-year-old male donor (filled circles). Little or no reactivity was observed when the primary antibody was omitted (open circles). Extracts prepared from the neural retina (filled triangles) of the same donor eye did not show a dose-dependent response when incubated with the A11 antibody. Dr, drusen.