Role of IFN-γ in induction of Foxp3 and conversion of CD4⁺ CD25^– T cells to CD4⁺ Tregs
J. Clin. Invest. Zhaojun Wang, et al. 116:2434 doi:10.1172/JCI25826 [Go to this article.]

Figure 6
Suppressing effect of IFN-γ–treated CD4⁺ CD25^– T cells in EAE. CD4⁺CD25^– T cells were purified from GKO mice and cultured in vitro with or without recombinant IFN-γ for 72 hours in the presence of anti-CD3 antibody. The resulting CD4⁺CD25^high T cells from both groups were purified and subsequently transferred to GKO mice (2 × 10⁶ cells per mouse) at day 9 and day 11 after immunization. CD4⁺CD25⁺ T cells purified from naive WT mice (Tregs) were transferred at the same cell number (2 × 10⁶ cells per mouse) and under the same experimental conditions without IFN-γ. GKO mice given PBS instead of cells were included as a reference (PBS). Each group consisted of at least 4 mice. Mice were monitored clinically every day for EAE development and progression. The data are presented as EAE clinical scores.