Role of IFN-γ in induction of Foxp3 and conversion of CD4⁺ CD25^– T cells to CD4⁺ Tregs
J. Clin. Invest. Zhaojun Wang, et al. 116:2434 doi:10.1172/JCI25826 [Go to this article.]

Figure 3
Encephalitogenic T cell response and cytokine profile in EAE mice. (A) Splenocytes isolated from GKO (IFN-γ^–/–) and WT (IFN-γ^+/+) mice at day 16 after immunization were examined for proliferation in the presence and absence (medium control) of the MOG peptide (5 μg/ml). Data are presented as mean cpm ± SD of triplicates. (B) Supernatants were collected from the above-mentioned culture after 48 hours and measured for the concentration of the indicated cytokines using ELISA. Values represent mean concentrations (pg/ml ± SD) of triplicates. Results were reproduced in 3 independent experiments. Asterisks represent statistical differences between groups; *P < 0.05.