Cardiac mast cell–derived renin promotes local angiotensin formation, norepinephrine release, and arrhythmias in ischemia/reperfusion
J. Clin. Invest. Christina J. Mackins, et al. 116:1063 doi:10.1172/JCI25713 [
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Figure 6The release of mast cell renin in isolated mouse hearts subjected to ischemia/reperfusion is associated with NE release and reperfusion arrhythmias. Nineteen mouse hearts were subjected to 20-minute ischemia (i.e., perfusion with glucose- and pyruvic acid–free KH buffer bubbled with 95% N
2 + 5% CO
2) followed by a 30-minute reperfusion with oxygenated KH buffer, either in the absence (
n = 7) or presence of cromolyn (300 μM;
n = 6) or BILA2157 (100 nM;
n = 6). Bars indicate means α SEM. (
A) Duration of reperfusion arrhythmias (VT/VF). Note that reperfusion arrhythmias were prevented in hearts pretreated with BILA2157. (
B and
C) Overflow of NE and Ang I–forming activity collected over 30 minutes either before ischemia or at the start of reperfusion. *Significantly different from own control;
P < 0.05 by 1-way ANOVA with Dunnett’s produre.
