Hemojuvelin is essential for dietary iron sensing, and its mutation leads
to severe iron overload
J. Clin. Invest. Vera Niederkofler, et al. 115:2180 doi:10.1172/JCI25683 [
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Figure 3Lack of
hepcidin expression in
Hjv-mutant mice. (
A) Developmental time course (E13.5–P90) of
Hjv,
hepcidin, and
GAPDH expression levels as determined by Northern blot analysis on total RNA isolated from liver. (
B) Northern blot analysis of total RNA isolated from adult (P90) or P1.5 liver of wild-type and
Hjv–/– mice probed for the expression of
Hjv,
hepcidin, and
GAPDH. (
C) Northern blot analysis of total RNA isolated from adult (P90) liver of wild-type and
Hjv–/– mice sacrificed 7 days after sham injection (S) or injection with iron-dextran (ID) and probed for the expression of
hepcidin and
GAPDH. Scale bar: 100 μm. (
D) In situ hybridization on cryostat sections of liver isolated from adult (P90) wild-type and
Hjv–/– mice probed for the expression of
hepcidin.
