Mll partial tandem duplication induces aberrant Hox expression in vivo via specific epigenetic alterations
J. Clin. Invest. Adrienne M. Dorrance, et al. 116:2707 doi:10.1172/JCI25546 [
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Figure 3Mll WT and
Mll PTD transcripts.
Schematic demonstrating the real-time RT-PCR strategy for detection and absolute quantification of the
Mll WT and
Mll PTD transcripts in bone marrow and spleen of
Mll PTD mice. The predicted
Mll PTD and
Mll WT allele–derived transcripts are shown, with the tandemly duplicated exons 5–11 present in the PTD transcript denoted with light gray boxes. Shown below the transcripts are sites for PCR primers and fluorogenic probes designed to amplify either the unique exon 11–exon 5 fusion (open rectangle) or exons 14–15, whose junction is common to the
Mll WT and
Mll PTD transcripts (filled rectangle).
