Mll partial tandem duplication induces aberrant Hox expression in vivo via specific epigenetic alterations
J. Clin. Invest. Adrienne M. Dorrance, et al. 116:2707 doi:10.1172/JCI25546 [Go to this article.]

Figure 2
Germline transmission and verification of correct targeting in mice heterozygous for the Mll PTD. (A) Southern blot analysis using high molecular-weight DNA from spleens, digested with NdeI and hybridized to a probe that spans intron 4 and exon 5 of Mll (i4e5 in Figure 1). This generates a single WT band at 18 kb in Mll^WT/WT mice, 2 bands representing 1 WT allele at 18 kb, and a band at 40 kb representing the rearranged allele in Mll^PTD/WT mice. (B) The Mll PTD fusion transcript was amplified using an upstream primer from Mll exon 11 and a downstream primer from Mll exon 6 amplifying a single 335-bp unique fusion transcript in the Mll^PTD/WT mouse (+) and is absent in the sample from the Mll^WT/WT mouse (–). Sequencing of these PCR products from the Mll^PTD/WT mice verified the presence of the exon 11–exon 5 fusion site. The 1-kb+ ladder was used to determine amplification size.