Cell-derived anaphylatoxins as key mediators of antibody-dependent type II autoimmunity in mice
J. Clin. Invest. Varsha Kumar, et al. 116:512 doi:10.1172/JCI25536 [Go to this article.]

Figure 6
FcγR-dependent C5a production and induction of C5 mRNA synthesis in Kupffer cells but not SECs in anemia. (A and B) Supernatants of liver homogenates from WT mice collected at different time points (A) or from WT, C5aR^–/–, and FcγRI/III^–/– mice collected 24 hours after AIHA induction (B) were assayed for C5a-dependent chemotactic activity on PMNs derived from WT mice (black bars) or C5aR^–/– mice (negative control, gray bars). (C and D) Kupffer cell and SEC C5 mRNA levels of the indicated WT, FcγRI/III^–/–, and C5aR^–/– mice at 4 hours after injection of 34-3C mAb (black bars) as compared with mice not receiving 34-3C mAb (gray bars). (A–D) Results are expressed as mean values ± SD obtained from 3–5 mice in each group. Significant differences were determined by Student’s t test (**P < 0.001).