Cell-derived anaphylatoxins as key mediators of antibody-dependent type II autoimmunity in mice
J. Clin. Invest. Varsha Kumar, et al. 116:512 doi:10.1172/JCI25536 [Go to this article.]

Figure 3
Reduced Kupffer cell–mediated rbc destruction in FcγRI/III^–/– and C5aR^–/– mice during 34-3C mAb–induced AIHA. (A) WT C57BL/6 (open circles), FcγRI/III^–/– (filled circles), and C5aR^–/– (filled squares) mice injected with 300 μg of anti-rbc 34-3C mAb were analyzed for Kupffer cell–mediated in vivo phagocytosis at the indicated times. Results are shown as the percentage of Mac-1–selected Kupffer cells containing more than 2 ingested rbcs ± SD obtained from 4–9 mice in each group. (B) H&E-stained liver sections obtained from the indicated mice at days 0 and 2 after AIHA induction were analyzed for erythrophagocytosis by light microscopy. Shown is the mean cell count ± SEM of rbc-containing liver cells per microscopic field (original magnifications, ×40 and ×100) obtained from 3–5 mice in each group. (A and B) Significant differences were determined by Student’s t test (*P < 0.05; **P < 0.001).