The LXXLL motif of murine forkhead transcription factor FoxO1 mediates Sirt1-dependent transcriptional activity
J. Clin. Invest. Jun Nakae, et al. 116:2473 doi:10.1172/JCI25518 [Go to this article.]

Figure 6
Nicotinamide inhibits FoxO1-dependent induction of a reporter construct under the control of an Igfbp-1 or G6Pase promoter. After hepatocytes were transfected with Igfbp-1/(p925GL3) (A) or G6Pase/luciferase reporter gene (PicaGene/human G6Pase promoter-luciferase) (B), cells were infected with indicated adenovirus. phRL-SV40 was used as an internal control of transfection efficiency. After overnight serum deprivation (black bars) and induction with dexamethasone/8-Br-cAMP/ IBMX as described in Methods in the presence (light gray bars) or absence (dark gray bars) of nicotinamide (50 mM), cells were harvested and luciferase activity was measured. *Statistically significant difference between luciferase activity in the absence and in the presence of nicotinamide in cells transfected with each vector. P < 0.005 by 1-way ANOVA. Data represent mean ± SEM from 3 independent experiments.