The LXXLL motif of murine forkhead transcription factor FoxO1 mediates Sirt1-dependent transcriptional activity
J. Clin. Invest. Jun Nakae, et al. 116:2473 doi:10.1172/JCI25518 [Go to this article.]

Figure 4
Effects of 3A/LXXAA FoxO1 on endogenous Igfbp-1 gene expression in SV40-transformed hepatocytes. (A) Expression of Flag 3A/LXXAA FoxO1 and HA-ADA FoxO1 at indicated MOI in SV40-transformed hepatocytes. (B) The effects of 3A/LXXAA FoxO1 on HA-ADA–induced Igfbp-1 gene expression in the presence of dexamethasone/8-Br-cAMP/IBMX in SV40-transformed hepatocytes. Data from 3 independent experiments are expressed as mean ± SEM of the folds of endogenous Igfbp-1 gene expression in the absence of HA-ADA FoxO1. *Statistically significant difference between 3A/LXXAA FoxO1 at MOI 0 and at MOI 10 or 20. P < 0.001 by 1-way ANOVA.