TRPA1 induced in sensory neurons contributes to cold hyperalgesia after inflammation and nerve injury
J. Clin. Invest. Koichi Obata, et al. 115:2393 doi:10.1172/JCI25437 [
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Figure 6Anti-NGF and a p38 MAPK inhibitor suppress cold hyperalgesia and TRPA1 upregulation in the spared L4 DRG neurons caused by injury to the L5 spinal nerve. (
A) Dark-field photomicrograph of ISHH showing the expression of TRPA1 and TRPM8 mRNA in the naive DRG, the injured L5 DRG, and the intact L4 DRG at day 7 after L5 SNL. (
B) Cold hyperalgesia was determined using the cold plate test at days 3 and 7 after L5 SNL. Data represent mean ± SEM;
n = 8 per group. (
C and
D) Quantification of the percentage of TRPA1 (
C) and TRPM8 (
D) mRNA–positive neurons at day 7 after surgery. Data represent mean ± SD;
n = 4 per group. *
P < 0.05 compared with the naive control;
#P < 0.05 compared with the vehicle group. Scale bar: 100 μm.
