TRPA1 induced in sensory neurons contributes to cold hyperalgesia after inflammation and nerve injury
J. Clin. Invest. Koichi Obata, et al. 115:2393 doi:10.1172/JCI25437 [
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Figure 4Anti-NGF and a p38 MAPK inhibitor reverse cold hyperalgesia and TRPA1 upregulation induced by inflammation. (
A) Cold and heat hyperalgesia were tested using the cold plate test and the plantar test, respectively, at days 1 and 3 after CFA injection. Mechanical allodynia was determined with a Dynamic Plantar Aesthesiometer. Data represent mean ± SEM;
n = 8 per group. (
B and
C) Quantification of the percentages of TRPA1 (
B) and TRPM8 (
C) mRNA–positive neurons at day 3 after CFA injection. Data represent mean ± SD;
n = 4 per group. (
D) Double labeling for TRPA1 or TRPM8 mRNA and p-p38–IR at day 3 after CFA injection. Open arrows indicate double-labeled neurons. SB, SB203580. *
P < 0.05 compared with the naive control;
#P < 0.05 compared with the vehicle group. Scale bar: 50 μm.
