Anaphylactic shock depends on PI3K and eNOS-derived NO
J. Clin. Invest. Anje Cauwels, et al. 116:2244 doi:10.1172/JCI25426 [
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Figure 3NO that causes PAF shock is produced by eNOS, activated via PI3K. (
A) C57BL/6 (WT), iNOS
–/–, or eNOS
–/– mice were injected i.v. with 55 μg PAF after pretreatment with vehicle or WM. Survival is presented as a combined Kaplan-Meier plot of 2 independent, representative experiments. The total number of mice is indicated between brackets. ***
P < 0.0001 compared with PAF in WT mice. (
B) Western blot for phosphorylated eNOS (p-eNOS) in kidney homogenates of individual mice 7 or 15 minutes after PAF injection. Where indicated, mice were pretreated with WM.
