BLyS and APRIL in rheumatoid arthritis
J. Clin. Invest. Thorsten M. Seyler, et al. 115:3083 doi:10.1172/JCI25265 [Go to this article.]

Figure 2
Cellular origin of APRIL and BLyS in rheumatoid synovitis. Frozen tissue sections were double stained with either anti-APRIL and anti-CD83 or anti-BLyS and anti-CD68. (A) APRIL was visualized with VectorBlue, and CD83 was detected with VectorRed. APRIL was exclusively produced by activated DCs localized in the T cell zones. (B) Binding of anti-BLyS was developed with 3,3′-diaminobenzidine tetrahydrochloride (brown staining), and CD68 expression was demonstrated with VectorRed. BLyS derived exclusively from CD68⁺ macrophages. Immunohistochemistries shown are representative of 20 experiments. Original magnification, ×600 (right panels in B). Scale bars: 200 μm (A); 50 μm (B).