Blocking airway mucous cell metaplasia by inhibiting EGFR antiapoptosis and IL-13 transdifferentiation signals
J. Clin. Invest. Jeffrey W. Tyner, et al. 116:309 doi:10.1172/JCI25167 [Go to this article.]

Figure 1
Persistent EGFR activation on ciliated epithelial cells after viral infection. (A) Representative photomicrographs of airway sections from C57BL/6J mice obtained at 21 days after inoculation with SeV or an equivalent amount of SeV-UV and then immunostained for EGFR and p-EGFR as well as competition by 50-fold antigen (Ag) excess. Scale bar: 20 μm. (B) Representative photomicrographs of airway sections obtained from mice at 21 days after inoculation with SeV and then subjected to immunofluorescent staining for EGFR, β-tubulin, CCSP, and MUC5AC alone and in combination. Primary anti-EGFR Ab binding was detected by anti-CY3 Ab (red fluorescence) while others were detected by anti-FITC Ab (green fluorescence). Scale bar: 20 μm. Similar results were obtained for mice treated with SeV-UV.