Type 2 iodothyronine deiodinase is the major source of plasma T3 in euthyroid humans
J. Clin. Invest. Ana Luiza Maia, et al. 115:2524 doi:10.1172/JCI25083 [
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Figure 8The T
3 derived from D2-catalyzed T
4 to T
3 conversion is more potent than that from D1 in stimulating T
3-dependent gene expression. (
A and
B) TRE3TKLUC induction in D1- and D2-expressing HEK 293 cells. At any rate of T
3 production (see tables below graphs), D2-catalyzed T
3 production causes greater increases in T
3-dependent luciferase (LUC) expression than T
3 derived from the D1-catalyzed T
4 to T
3 reaction. (
C) TRE3TKLUC induction versus T
3 production (log
10). D2-derived T
3 caused greater LUC induction than did D1-derived T
3. LUC expression was normalized to growth hormone as transfection efficiency control. (
D) Effects of added T
3 and D1- or D2-derived T
3 on TRE3TKLUC induction. Cells transfected with inactive D2 were incubated with 1 ml 0.1% BSA DMEM, and the indicated amounts of T
3 were added. D1- and D2-expressing cells were incubated in the same medium containing T
3-Ab (1:1,000) and 20 pM FT
4.
