Type 2 iodothyronine deiodinase is the major source of plasma T3 in euthyroid humans
J. Clin. Invest. Ana Luiza Maia, et al. 115:2524 doi:10.1172/JCI25083 [
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Figure 7A T
3-responsive system for evaluating the contribution of D1- and D2-generated T
3 to nuclear T
3. (
A–
C) HEK 293 cells were transfected with D1- or D2-expressing plasmids, a TRα-expressing plasmid, and the T
3-responsive TRE3TKLUC construct (see Methods). (
A) The TRE3TKLUC reporter responded to T
3 in a dose-dependent manner. (
B) The addition of specific T
3-Ab prevents cellular uptake of T
3 but not T
4. (
C) T
3-Ab blocks the transcriptional response to 20 pM T
3.
