Small-molecule correctors of defective ΔF508-CFTR cellular processing identified by high-throughput screening
J. Clin. Invest. Nicoletta Pedemonte, et al. 115:2564 doi:10.1172/JCI24898 [Go to this article.]

Figure 3
Increased ΔF508-CFTR sensitivity to activators after incubation with correctors. (A) Effect of forskolin (20 μM) or forskolin plus genistein (50 μM) in ΔF508-CFTR–expressing FRT cells kept at 37°C or 27°C with or without correctors. (B) Forskolin dose-response relationships. Cells stimulated with forskolin (in the absence of genistein) after incubation with correctors (at 37°C) or at 27°C. (C) Apical membrane chloride current after incubation for 24 hours at 37°C with DMSO vehicle (left curve) or corr-2b (middle curves). The curve at the far right was obtained from an experiment on cells grown at 27°C with compounds added as shown (20 μM forskolin, 20 μM corr-2b, 50 μM genistein).