Alterations in regulation of energy homeostasis in cyclic nucleotide phosphodiesterase 3B–null mice
J. Clin. Invest. Young Hun Choi, et al. 116:3240 doi:10.1172/JCI24867 [
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Figure 6Differences in insulin secretion from pancreatic islets, serum insulin concentrations, and blood glucose disposal in WT and
Pde3b-KO mice.
(
A) Insulin accumulation during incubation of isolated pancreatic islets for 1 hour with 3 or 16.7 mM glucose and without or with 100 nM GLP-1 as indicated. Data are mean ± SEM (
n = 4). Inset: Immunoreactive PDE3B was detected by Western blots of WT, not KO, homogenates. (
B) CL (1.0 mg/kg) in PBS or PBS alone was injected i.p. (10 ml/kg) into 6-month-old WT and KO mice. At the indicated times, differences from time 0 (basal) in serum insulin levels were quantified. Basal insulin levels in WT and KO mice were 1.4 ± 0.2 and 2.6 ± 0.1 ng/ml, respectively. Data (mean ± SEM;
n = 4 mice per group) are representative of 3 experiments. (
C and
D) ISO or CL (1.0 mg/kg) in PBS or PBS alone were injected i.p. or i.v. (10 ml/kg) into 3- (i.p) or 4-month-old (i.v.) WT and KO mice. After 20 minutes, tail blood was collected, and changes from basal serum insulin (
C) and blood glucose (
D) levels were measured. Basal values were similar in PBS-treated WT and KO mice. Data (mean ± SEM;
n = 4–5 mice per group) are representative of 3 experiments. *
P < 0.05; **
P < 0.01;
†P < 0.001.
