A frameshift polymorphism in P2X5
elicits an allogeneic cytotoxic T lymphocyte response associated with remission
of chronic myeloid leukemia
J. Clin. Invest. Björn de Rijke, et al. 115:3506 doi:10.1172/JCI24832 [
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Figure 4Detection of LRH-1–specific CD8
+ T cells in peripheral blood of CML patient UPN389 after DLI. PBMCs collected 3, 6, 12, and 38 months after DLI-2 were stained with LRH-1/HLA-B7 tetramer, anti-CD8, anti-CD45, anti-CD3, and 7-amino-actinomycin D (7AAD). Subsequently, cell populations were analyzed by flow cytometry. Cells were gated on CD45
+CD3
+7AAD
– lymphocytes, and the percentages of tetramer-binding cells among CD8
+ T cells are given. The remaining PBMCs were stimulated twice with LRH-1 peptide–pulsed (10 μM) EBV-LCL of the donor and assayed on day 14 for LRH-1 tetramer-binding CD8
+ T cells.
