Integration of flow-dependent endothelial phenotypes by Kruppel-like factor 2
J. Clin. Invest. Kush M. Parmar, et al. 116:49 doi:10.1172/JCI24787 [
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Figure 4KLF2 mediates a global antiinflammatory program in endothelial cells. (
A) Protein levels of cytokines in supernatants of HUVECs infected with the indicated virus and then incubated with either normal media or IL-1β (10 U/ml) for 24 hours. Samples were analyzed by multiplex ELISA or cytokine chip. IP-10, IFN-γ–inducible protein 10. (
B) Regulation of PTGDS gene expression by KLF2 and/or IL-1β. (
C) ELISA of 15d-PGJ2 after extraction from supernatants of cells infected with the indicated virus for 24 hours. (
D) ELAFIN secretion measured by ELISA from supernatants under conditions described in
A. All data are expressed as mean ± SEM (
n = 3). *
P < 0.05, **
P < 0.01 vs. control; Student’s
t test.
