c-Fos protein as a target of anti-osteoclastogenic action of vitamin D, and synthesis of new analogs
J. Clin. Invest. Hisashi Takasu, et al. 116:528 doi:10.1172/JCI24742 [
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Figure 8Screening for vitamin D (VD) analogs that reduce c-Fos protein and inhibit osteoclast differentiation more potently than 1α,25(OH)
2D
3.
The effects of 1α,25(OH)
2D
3 (open circles) and its analogs (DD281, DD280, and DD208) on the c-Fos protein level in osteoclast precursor cells (
A and
B) and the formation of TRAP-positive multinucleate osteoclasts (
C) at the indicated concentrations are shown. The lower bands in
A show β-actin as an internal control for protein loading. Filled circles, rectangles, and triangles (
B and
C) represent DD281, DD280, and DD208, respectively. Data are expressed as a percentage of the value for vehicle-treated cultures.
