Combretastatin A4 phosphate induces rapid regression of tumor neovessels and growth through interference with vascular endothelial-cadherin signaling
J. Clin. Invest. Loïc Vincent, et al. 115:2992 doi:10.1172/JCI24586 [Go to this article.]

Figure 3
CA4P induces retraction of endothelial cells and disrupts actin cytoskeleton. (A) CA4P induces retraction of endothelial cells. The effect of CA4P (5 nM and 10 nM) on the cell shape of FGF-2–stimulated HUVECs was monitored during 48 hours by microscopy. Notice the retraction of HUVECs after 18 hours of treatment with CA4P. Magnification: ×20. Scale bar, 10 μm. (B) The actin cytoskeleton was stained with TRITC-labeled phalloidin and analyzed by fluorescent microscopy on FGF-2–stimulated HUVECs after an 18-hour incubation with CA4P. Addition of CA4P resulted in reorganization of actin stress fibers. Magnification: ×40. Scale bar, 5 μm.