Inactivation of focal adhesion kinase in cardiomyocytes promotes eccentric cardiac hypertrophy and fibrosis in mice
J. Clin. Invest. Xu Peng, et al. 116:217 doi:10.1172/JCI24497 [
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Figure 5Cardiac hypertrophy in older CFKO mice. (
A) The hearts of 9-month-old CFKO and control mice were harvested. The total ventricular RNA was isolated and subjected to Northern blotting with various probes, as indicated. (
B) The intensity of the bands was quantified from 3 independent experiments by densitometry. The mean ± SD values of relative intensity (normalized to the control mice) are shown in arbitrary units. *
P < 0.05 compared with values from control mice. (
C and
D) Cardiomyocytes were isolated from CFKO and control mice as described in Methods. They were then analyzed for area (
C) and for length of long and short axes (
D) using Image-Pro Plus version 3.0 morphometric analysis software. The mean ± SD values from 3 independent experiments are shown. *
P < 0.05 compared with values from control mice. Representative images of isolated cardiomyocytes are also shown in
C.
