Ets-1 is a critical regulator of Ang II-mediated vascular inflammation and remodeling
J. Clin. Invest. Yumei Zhan, et al. 115:2508 doi:10.1172/JCI24403 [Go to this article.]

Figure 2
Effect of Ang II on systolic blood pressure and [³H]leucine and [³H]thymidine incorporation in cultured primary aortic SMCs isolated from Ets1^+/+ and Ets1^–/– mice. (A) Quiescent aortic SMCs were cultured in the presence of varying concentrations of Ang II (0, 30, 100, and 300 nM) for 18 hours and pulsed with [³H]leucine for 6 hours. Incorporation was measured by a liquid scintillation spectrophotometer. Experiments were repeated 3 times. Values represent mean ± SEM. *P < 0.05, **P < 0.01 versus unstimulated control cells; ^†P < 0.05; ^#P < 0.01. (B) Effect of Ang II on [³H]thymidine uptake. Quiescent aortic SMCs were stimulated with different concentrations of Ang II for 18 hours and pulsed with [³H]thymidine for 5 hours. Incorporation of [³H]thymidine was measured by a liquid scintillation spectrophotometer. Experiments were repeated 3 times. Values represent mean ± SEM. *P < 0.05, **P < 0.01 versus unstimulated control cells; ^#P < 0.01. (C) Blood pressure of mice was measured using a Visitech BP-2000 system (see Methods) before (Pre) and after Ang II infusion (1.4 mg/kg/d) for 1 week and 2 weeks. **P < 0.01 versus SMCs before Ang II infusion.