Carcinoembryonic antigen–related cell adhesion molecule 1 modulates vascular remodeling in vitro and in vivo
J. Clin. Invest. Andrea Kristina Horst, et al. 116:1596 doi:10.1172/JCI24340 [Go to this article.]

Figure 2
Generation and characterization of CEACAM1^endo+ mice. (A) Transgenic construct for the generation of CEACAM1^endo+ transgenic mice. The Ceacam1-L cDNA with an additional SV40 polyadenylation (pA) signal was cloned into the Sse8387 I and Mlu I restriction sites, downstream of the Tie2 promoter and upstream of a Tie2 intronic enhancer. The arrow indicates the position and length of the probe used for genotyping transgenic mice by Southern blots and the fragment that was amplified by PCR. (B) Southern blot of CEACAM1^endo+ transgenic founder lines. Microinjection of oocytes with the construct depicted in A yielded 5 founder lines, as shown by Southern Blot analysis. Con, control. (C) Confirmation of endothelial CEACAM1 overexpression on primary endothelial cells from lung by flow cytometric analysis using anti-PECAM1 and anti-CEACAM1 double labeling in CEACAM1^endo+ mice.