In vivo expansion of CD4⁺CD45RO–CD25⁺ T cells expressing foxP3 in IL-2-treated HIV-infected patients
J. Clin. Invest. Irini Sereti, et al. 115:1839 doi:10.1172/JCI24307 [Go to this article.]

Figure 6
CEN cells express high levels of foxP3. (A) Levels of foxP3 mRNA by RT-PCR in CD4⁺ T cells, from a representative IL-2–treated patient, separated by CD45RO and CD25 into 4 subsets (CD45RO^–CD25^– and CD25⁺, CD45RO⁺CD25^– and CD25⁺ [low and high]). (B) Real-time PCR was used to study the relative expression of foxP3 in CD45RO^–CD25⁺ versus CD45RO⁺CD25⁺ cells. For 5 independent donors (IL-2–treated patients; diamonds), foxP3 expression was higher in the CD45RO^–CD25⁺ subset than in the CD45RO⁺CD25⁺ subset (including cells with both low and high CD25 expression). Median values are shown as black bars. (C) foxP3 expression in CD4⁺CD45RO^–CD25⁺ cells and CD4⁺CD45RO⁺CD25^+high cells from IL-2–treated patients (diamonds, median values as black bars) is similar to that observed in identical CD4 subsets from healthy volunteers (circles, median values as gray bars).