In vivo expansion of CD4⁺CD45RO–CD25⁺ T cells expressing foxP3 in IL-2-treated HIV-infected patients
J. Clin. Invest. Irini Sereti, et al. 115:1839 doi:10.1172/JCI24307 [Go to this article.]

Figure 5
CEN cells require costimulation for proliferation and can weakly suppress CD4⁺CD45RO^–CD25^– cells. (A) Separated CEN (black bars) or naive CD25^– cells (white bars) from IL-2–treated patients were cultured in the presence of media, anti-CD3, or PHA for 96 hours. Media (background) proliferation was subtracted. Median values from 9 independent experiments using different donors are shown. (B) Separated CD4⁺CD25^– cells were cultured at fixed numbers either alone (white bars) or in the presence of CD4⁺CD25⁺ cells at ratios of 1:2 (black bars) or 1:1 (gray bars). Median values from 7 independent experiments using different donors are shown.