Atrial natriuretic peptide promotes cardiomyocyte survival by cGMP-dependent nuclear accumulation of zyxin and Akt
J. Clin. Invest. Takahiro Kato, et al. 115:2716 doi:10.1172/JCI24280 [
Go to this article.]
Figure 6Elevation of cGMP induces rapid nuclear accumulation of activated Akt kinase. cGMP and ANP increased the expression level of phospho-Akt in the nuclear fraction. Cells were labeled with phalloidin (
A, left side) to reveal sarcomeric organization. Confocal microscopy shows the nuclear accumulation of phospho-Akt increase in the cardiomyocyte with cGMP (10
–4 M) or ANP (10
–9 M) treatment (
A, middle). Actin filament (red) and zyxin (green) are depicted in overlay panels (
A, right side). Scale bars, 60 μm. (
B and
C) Bar graph shows the ratio of the nuclear accumulation of phospho-Akt with cGMP (10
–4 M) or ANP (10
–9 M) treatment obtained from confocal microscopic analysis.
n = 3. *
P < 0.05.
