Atrial natriuretic peptide promotes cardiomyocyte survival by cGMP-dependent nuclear accumulation of zyxin and Akt
J. Clin. Invest. Takahiro Kato, et al. 115:2716 doi:10.1172/JCI24280 [Go to this article.]

Figure 2
ANP induces nuclear accumulation of zyxin at antiapoptotic concentration. Immunolabeling (A and B) and quantitation of nuclear labeling (C and D) in cultured cardiomyocytes treated with ANP (10^–9 for 1 hour for A, B, and D; 10^–6 M for 1 hour also shown for B). Immunolabeling at high (A) and low (B) magnification shows zyxin (green in overlay) predominantly associated with myofibrillar striations and focal adhesion regions in periphery of untreated cells (control). 10^–9 M ANP treatment resulted in redistribution and nuclear accumulation of zyxin whereas nuclear accumulation did not occur at 10^–6 M ANP. Myofibrillar organization was observed with phalloidin to label actin filaments (red in overlay). Bar measurements in micrographs represent distance in μm. Quantitation (C) shows dosage-dependent nuclear accumulation of zyxin peak at 10^–9 M ANP 2 hours after treatment. Time course of nuclear zyxin accumulation (D) shows peak level within hours after treatment and return to normal level within 24 hours. n = 3 for all experiments. *P < 0.01.