Developmental control of CD8⁺ T cell–avidity maturation in autoimmune diabetes
J. Clin. Invest. Bingye Han, et al. 115:1879 doi:10.1172/JCI24219 [Go to this article.]

Figure 5
Functional avidity and diabetogenic potential of peripheral IGRP_206–214 tetramer–reactive cells from 17.4α/8.3β-Tg versus 17.5α/8.3β-Tg animals. (A) Proliferation of purified CD8⁺ splenocytes (adjusted for the number of IGRP_206–214 tetramer⁺ cells) to IGRP_206–214 in the presence of irradiated NOD splenic APCs. Splenic T cells were isolated from 5- to 7-week-old animals. (B) Insulitis scores in prediabetic mice (n = 9, 7.3 ± 0.7-week-old for 17.4α/8.3β-Tg mice; and n = 2, 9-week-old for 17.5α/8.3β-Tg mice). (C) Cumulative incidence of diabetes in 214 (17.4α/8.3β-Tg) and 53 (17.5α/8.3β-Tg) RAG-2⁺ mice. (D) Cumulative incidence of diabetes in 73 (17.4α/8.3β-Tg) and 8 (17.5α/8.3β-Tg) RAG-2^–/– mice. (E) Representative splenic flow cytometry profiles (CD4, CD8, and Vβ8) of RAG-2^–/– 17.4α/8.3β-Tg versus 17.5α/8.3β-Tg mice.