Developmental control of CD8⁺ T cell–avidity maturation in autoimmune diabetes
J. Clin. Invest. Bingye Han, et al. 115:1879 doi:10.1172/JCI24219 [Go to this article.]

Figure 4
Striking differences in the developmental biology of 17.4α/8.3β and 17.5α/8.3β T cells in Tg mice. Flow cytometry of thymocytes (A–D) and splenocytes (E–H). (A and E) Representative CD4 versus CD8 dot plots. P values for percentage of thymic and splenic CD8⁺CD4^– cells were as follows: P < 0.017 (for 17.4α/8.3β Tg vs. 17.5α/8.3β Tg, 5- to 9-week-old mice). (B–D and F–H) Comparison of mfi with which the different T cell subsets stain with anti–TCR-Vβ8 or anti–TCR-β mAbs or with IGRP_206–214 tetramers. Values on the panels correspond to percentage of cells contained within each quadrant (A and E) or to percentage of cells within each gate that stained (B–D and F–H) (mean ± SE). **P < 0.05 versus 17.4α/8.3β Tg mice. Representative histograms are shown. A–D and E–H are representative of 4–5 mice per strain per age group. Note that the representative dot plots and histograms corresponding to 17.4α/8.3β Tg mice are the same as those shown in Figure 2. The data are rendered twice to facilitate comparisons with results obtained with Tg mice bearing lower- (Figure 2) or higher-avidity T cells (this figure).