Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display
J. Clin. Invest. Aimee S. Payne, et al. 115:888 doi:10.1172/JCI24185 [Go to this article.]

Figure 7
Epitope mapping of (D3)3c/9 and (D31)2/28 against mouse and human desmogleins. Wild-type and domain-swapped extracellular domains of mDsg1 and mDsg3 (lanes 1–6) or wild-type extracellular domains of human desmogleins (hDsgs) 1, 3, and 4 (lanes 7–9) were produced in baculovirus and immunoprecipitated with scFv mAbs. Desmoglein proteins bound by human scFv mAbs were detected by immunoblot analysis using antibody against E tag, which was engineered onto the carboxyterminal domain of recombinant desmoglein molecules. The top panel shows the immunoblot of the recombinant and chimeric desmogleins detected with anti–E tag. The bottom 2 panels show the results of the immunoprecipitation-immunoblotting studies.