Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display
J. Clin. Invest. Aimee S. Payne, et al. 115:888 doi:10.1172/JCI24185 [Go to this article.]

Figure 4
Epitopes recognized by PV scFv mAbs are blocked by sera from multiple pemphigus patients. (A) (D3)3c/9. Left: Inhibition of Dsg3 binding by all 8 PV sera. PVLIB is the plasma of the mucocutaneous PV patient from whom the phage display library was made. Black bars represent PV sera containing anti-Dsg3 antibodies only; gray bars indicate PV sera containing antibodies with both anti-Dsg3 and anti-Dsg1 activity [PV(3+1) sera]. Right: Inhibition of Dsg1 binding by 3 of 5 PV(3+1) sera and 6 of 6 PF sera (white bars). (B) (D31)2/28. Left: Inhibition of Dsg3 binding by 7 of 8 PV sera and 0 of 6 PF sera. Right: Inhibition of Dsg1 binding by 4 of 5 PV(3+1) sera and 5 of 6 PF sera. Inhibition of greater than 20% was considered positive. NHS, normal human sera.