Heme-regulated eIF2α kinase modifies the phenotypic severity of murine models of erythropoietic protoporphyria and β-thalassemia
J. Clin. Invest. An-Ping Han, et al. 115:1562 doi:10.1172/JCI24141 [
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Figure 1HRI alters the hematological parameters of Fech
m1Pas/m1Pas mice. (
A) Activation of HRI in the reticulocytes of Fech-deficient mice. Activation of HRI in the blood samples of 2-week-old mice from the same litter was analyzed by SDS-PAGE and Western blot analysis using anti-HRI antibody (top panel). The in vivo phosphorylation of eIF2α (eIF2αP) and the total eIF2α in the blood samples of various mice were determined by Western blot analysis using antibodies specifically against phosphorylated eIF2α (middle panel) and eIF2α (lower panel). (
B) The complete blood analyses. Data are presented as mean ± SD. Retic, reticulocyte. Units of the hematological parameters: rbc, ×10
12/l; Hb, g/dl; MCV, fl; MCH, pg; retic, %; PPIX, arbitrary units. (
C) Examination of the blood smears by light microscopes. Original magnification, ×1,000. (
D) Examination of the blood smears by electron microscopes. Original magnification, ×22,000. Arrow indicates intraerythroid inclusions. The blood samples from 12- to 16-week-old mice were used for these analyses.
